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1. Quantities of 1 µg ACTH added to normal plasma were recovered after extraction by an acid-acetone technique, and after adsorption of the resultant extracts on to, and subsequent elution from, oxycellulose columns.

2. Adrenocorticotrophic activity has been demonstrated in acid-acetone extracts of plasma from patients with Addison's disease both with and without DCA or cortisone therapy, from a female pseudohermaphrodite, in two patients with post-operative stress, and in a patient in in diabetic coma.

3. All extracts were assayed in more than one dose, and the difficulties in obtaining valid assays against standard preparations are discussed.