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This study set out to elucidate the mechanism by which H₂O₂ generation is regulated in cultured porcine thyroid cells. We monitored continuously the effects of the calcium ionophore A23187 [GenBank] and the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA) on H₂O₂ generation, using homovanillic acid and horseradish peroxidase. A23187 [GenBank] and TPA stimulated H₂O₂ generation. A23187 [GenBank] increased cytoplasmic free calcium and TPA activated protein kinase C. Generation of H₂O₂ is therefore regulated by cytoplasmic free calcium and protein kinase C. Exposure to A23187 [GenBank] or TPA augmented further the stimulation of H₂O₂ generation by TPA or A23187 [GenBank] respectively. Thus A23187 [GenBank] and TPA, by increasing cytoplasmic free calcium and activating protein kinase C respectively, synergistically activate H₂O₂ generation.

Journal of Endocrinology (1989) 120, 503–508