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Journal of Endocrinology (1989) 122, 177-183    DOI: 10.1677/joe.0.1220177
© 1989 Society for Endocrinology
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Interleukin-1 inhibits thyrotrophin-induced human thyroglobulin gene expression

 S. Yamashita, H. Kimura, K. Ashizawa, Y. Nagayama, H. Hirayu, M. Izumi and S. Nagataki

The regulation of thyroglobulin (Tg) and its specific mRNA by interluekin-1 (IL-1) in cultured human thyrocytes was investigated. Specific binding of 125I-labelled IL-1 on thyrocytes was confirmed by solid-phase binding assay. Thyrocytes dispersed from Graves' thyroid tissues were incubated with TSH with or without recombinant human IL-1. TSH stimulated Tg release from cultured human thyrocytes in a dose- and time-dependent manner. Both IL-1{alpha} and β inhibited TSH-induced Tg release at concentrations ranging from 0·01 to 10 U/ml. The suppressive activities of IL-1{alpha} and β were similar. They did not alter the basal level of Tg release. Unstimulated human thyrocytes did not contain any detectable Tg mRNA, but TSH-stimulated thyrocytes expressed a single species of Tg mRNA (8·5 kb). Both IL-l{alpha} and β inhibited TSH-induced Tg mRNA in a dose-responsive manner. IL-1 (10 U/ml) caused maximal suppression of TSH-induced Tg mRNA to nearly basal levels. In contrast, the {gamma}-actin mRNA hybridization signal was not altered in control or treated cells. Furthermore, IL-1 stimulated [3H]thymidine uptake into thyrocyte DNA. These results demonstrate that IL-1 directly inhibits TSH-induced Tg gene expression and provide further support for a functional role of IL-1 as a local modulator of thyroid hormone synthesis.

Journal of Endocrinology (1989) 122, 177–183




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