HOME HELP CONTACT US SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Winder, S. J. Articles by Forsyth, I. A. Search for Related Content PubMed Articles by Winder, S. J. Articles by Forsyth, I. A.

Ovine mammary epithelial cell clumps (30–90 µm) were plated onto attached gels of rat tail collagen in serum-free medium. Synthesis of DNA by these cultures could be stimulated by insulin-like growth factor-I (IGF-I) with a median effective dose of 5 µg/l, irrespective of stage of pregnancy. The time-course of response, however, was significantly slower in cells prepared from mammary tissue of non-pregnant and early pregnant sheep compared with sheep later in pregnancy. IGF-II had approximately 10% of the potency of IGF-I in stimulating DNA synthesis. Insulin acted over a wide concentration range and produced a maximum rate of stimulation not significantly different from that produced by IGF-I. These results are consistent with actions through the type-I IGF receptor although insulin may also act through its own receptor, possibly stimulating local IGF-I production. It is concluded that IGF-I is an important mitogen for ovine mammary epithelial cells.

Journal of Endocrinology (1989) 123, 319–326

This article has been cited by other articles:

				S. H. Kang, J. U. Kim, J. Y. Imm, S. Oh, and S. H. Kim The Effects of Dairy Processes and Storage on Insulin-Like Growth Factor-I (IGF-I) Content in Milk and in Model IGF-I-Fortified Dairy Products J Dairy Sci, February 1, 2006; 89(2): 402 - 409. [Abstract] [Full Text] [PDF]

				S. M. Donovan, J. L. Hartke, M. H. Monaco, and M. B. Wheeler Insulin-like Growth Factor-I and Piglet Intestinal Development J Dairy Sci, July 1, 2004; 87(13_suppl): E47 - 54. [Abstract] [Full Text] [PDF]

				E. L. Annen, R. J. Collier, M. A. McGuire, and J. L. Vicini Effects of Dry Period Length on Milk Yield and Mammary Epithelial Cells J Dairy Sci, July 1, 2004; 87(13_suppl): E66 - 76. [Abstract] [Full Text] [PDF]

				R. C. Hovey, J. Harris, D. L. Hadsell, A. V. Lee, C. J. Ormandy, and B. K. Vonderhaar Local Insulin-Like Growth Factor-II Mediates Prolactin-Induced Mammary Gland Development Mol. Endocrinol., March 1, 2003; 17(3): 460 - 471. [Abstract] [Full Text] [PDF]