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Urinary gonadotrophins from amenorrhoeic patients were assayed. The results provide preliminary evidence that FSH and ICSH exist as separate entities in human urine. Cortisone treatment appeared to cause a rise in the excretion of FSH relative to ICSH. The reverse effect was caused by treatment with 0·5 mg of stilboestrol daily. Supplementary experiments suggested that prolactin and oestrogen had not interfered in the assays.