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1. In fasted adrenalectomized female rats a rise in blood sugar may be a more sensitive index of cortisone activity than a rise in liver glycogen.

2. There was a threshold level of blood sugar below which the liver was almost empty of glycogen. Above this threshold the concentration of liver glycogen rose almost logarithmically with a rise in blood sugar.

3. The shape of this blood sugar—liver glycogen relationship is discussed in relation to the bio-assay of corticosteroids and their effect on carbohydrate metabolism.

4. The concentration of glucose in the red cell of the rat is much less than that in the plasma.