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The intravenous injection of glucagon (0·4 mg/kg) 12–30 min before the administration of a diabetogenic dose of alloxan (60 mg/kg) prevents the development of alloxan diabetes in dogs.

The protection obtained with glucagon is abolished by a subcutaneous injection of insulin (2 i.u./kg) administered 2 hr previously.

The possible mechanism of this protection and of its antagonism by insulin is discussed.