JOE
HOME HELP CONTACT US SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Journal of Endocrinology (2000) 165, 39-49       DOI: 10.1677/joe.0.1650039
© 2000 Society for Endocrinology
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (12)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Pedchenko, V.
Right arrow Articles by Imagawa, W
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Pedchenko, V.
Right arrow Articles by Imagawa, W
Journal of Endocrinology, Vol 165, Issue 1, 39-49
Copyright © 2000 by Society for Endocrinology

Articles

Estrogen treatment in vivo increases keratinocyte growth factor expression in the mammary gland

VK Pedchenko and W Imagawa


Proliferation and differentiation of mammary epithelia are regulated by the combined action of systemic hormones and locally derived paracrine growth factors. Keratinocyte growth factor (KGF) is a potential candidate stromal factor that may participate in the hormonal control of stromal/epithelial interactions. In this study, we have examined the in vivo effect of 17beta-estradiol (E) treatment on KGF expression in mammary glands of peripubertal (5-week-old) and mature (11-week-old) mice. Mice received subcutaneous injections of hormone after which KGF mRNA levels were assayed by ribonuclease protection analysis of mammary gland RNA. E treatment caused a dose- and time-dependent increase in KGF mRNA levels in intact mice from both age groups. Neither 17alpha-estradiol nor progesterone injection affected KGF mRNA levels. Comparison of the relative expression of KGF in parenchyma-free fat pads and in intact glands demonstrated that the basal and E-dependent KGF mRNA levels did not require the presence of mammary epithelium. ELISA assay of KGF tissue content demonstrated that concomitantly with an up-regulation of mRNA, E treatment also increased KGF protein in mammary glands from peripubertal and mature mice. These data show that E treatment stimulates both KGF mRNA and protein expression in mammary stroma in vivo and raises the possibility that KGF has a role in E-regulated mammary gland development.


This article has been cited by other articles:


Home page
 Mol. Endocrinol.Home page
A. K. Nagaraja, C. Andreu-Vieyra, H. L. Franco, L. Ma, R. Chen, D. Y. Han, H. Zhu, J. E. Agno, P. H. Gunaratne, F. J. DeMayo, et al.
Deletion of Dicer in Somatic Cells of the Female Reproductive Tract Causes Sterility
Mol. Endocrinol., October 1, 2008; 22(10): 2336 - 2352.
[Abstract] [Full Text] [PDF]

Home page
 Endocr. Rev.Home page
R. H. Straub
The Complex Role of Estrogens in Inflammation
Endocr. Rev., August 1, 2007; 28(5): 521 - 574.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
H. Ka, S. Al-Ramadan, D. W. Erikson, G. A. Johnson, R. C. Burghardt, T. E. Spencer, L. A. Jaeger, and F. W. Bazer
Regulation of Expression of Fibroblast Growth Factor 7 in the Pig Uterus by Progesterone and Estradiol
Biol Reprod, July 1, 2007; 77(1): 172 - 180.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
H. Ka, L. A. Jaeger, G. A. Johnson, T. E. Spencer, and F. W. Bazer
Keratinocyte Growth Factor Is Up-Regulated by Estrogen in the Porcine Uterine Endometrium and Functions in Trophectoderm Cell Proliferation and Differentiation
Endocrinology, June 1, 2001; 142(6): 2303 - 2310.
[Abstract] [Full Text] [PDF]


HOME HELP CONTACT US SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2000 by the Society for Endocrinology.