JOE
HOME HELP CONTACT US SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Journal of Endocrinology (2000) 165, 483-492       DOI: 10.1677/joe.0.1650483
© 2000 Society for Endocrinology
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (29)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ochoa, A.
Right arrow Articles by Ben-Jonathan, N
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ochoa, A.
Right arrow Articles by Ben-Jonathan, N
Journal of Endocrinology, Vol 165, Issue 2, 483-492
Copyright © 2000 by Society for Endocrinology

Articles

Vascular endothelial growth factor in the rat pituitary: differential distribution and regulation by estrogen

AL Ochoa, NA Mitchner, CD Paynter, RE Morris, and N Ben-Jonathan


Vascular endothelial growth factor (VEGF), an endothelial cell mitogen and permeability factor, participates in tumor angiogenesis, but less is known about its regulation or function in normal vascular homeostasis. In the uterus, which undergoes cyclic changes in its vasculature, VEGF is induced by estrogen. Since the pituitary gland contains highly permeable capillaries and is estrogen-responsive, our objectives were to localize VEGF expression within the pituitary and to determine whether it is regulated by estrogen in both the pituitary and the somatolactotrope cell line, GH(3). Ovariectomized rats were injected with estradiol, and pituitaries and uteri were subjected to in situ hybridization or quantitative reverse transcription-polymerase chain reaction (RT-PCR). VEGF expression was strong and punctate in the neural lobe, weaker and diffuse in the anterior lobe and undetectable in the intermediate lobe. Two VEGF isoforms, 164 and 120, were detected in all tissues. In the posterior pituitary, VEGF expression was 3- to 6-fold higher than in the anterior pituitary or uterus and was unaltered by estrogen. In contrast, anterior pituitary VEGF was induced by estrogen within 1 h, peaked at 3 h, and returned to basal levels by 24 h. Similar dynamics, albeit 10-fold higher, were seen in the uterus. Translated VEGF proteins were detected by Western blot in both the anterior pituitary and uterus. GH(3) cells also showed a dose- and time-dependent induction of VEGF expression by estrogen. In conclusion: (1) VEGF expression is higher in the neural lobe than in the anterior lobe and is undetectable in the intermediate lobe, (2) the expression of VEGF164 and VEGF120 is rapidly upregulated by estrogen in the anterior pituitary but is unchanged in the posterior pituitary, and (3) the pituitary lactotrope cell line, GH(3), also increases VEGF expression in response to estradiol.


This article has been cited by other articles:


Home page
 EndocrinologyHome page
C. Cristina, G. Diaz-Torga, A. Baldi, A. Gongora, M. Rubinstein, M. J. Low, and D. Becu-Villalobos
Increased Pituitary Vascular Endothelial Growth Factor-A in Dopaminergic D2 Receptor Knockout Female Mice
Endocrinology, July 1, 2005; 146(7): 2952 - 2962.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
A. Lasorella, G. Rothschild, Y. Yokota, R. G. Russell, and A. Iavarone
Id2 Mediates Tumor Initiation, Proliferation, and Angiogenesis in Rb Mutant Mice
Mol. Cell. Biol., May 1, 2005; 25(9): 3563 - 3574.
[Abstract] [Full Text] [PDF]

Home page
 Mol Cancer ResHome page
K. Sengupta, S. Banerjee, N. K. Saxena, and S. K. Banerjee
Thombospondin-1 Disrupts Estrogen-Induced Endothelial Cell Proliferation and Migration and Its Expression Is Suppressed by Estradiol
Mol. Cancer Res., March 1, 2004; 2(3): 150 - 158.
[Abstract] [Full Text] [PDF]

Home page
 Endocr. Rev.Home page
H. E. Turner, A. L. Harris, S. Melmed, and J. A. H. Wass
Angiogenesis in Endocrine Tumors
Endocr. Rev., October 1, 2003; 24(5): 600 - 632.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
U. Renner, P. Lohrer, L. Schaaf, M. Feirer, K. Schmitt, C. Onofri, E. Arzt, and G. K. Stalla
Transforming Growth Factor-{beta} Stimulates Vascular Endothelial Growth Factor Production by Folliculostellate Pituitary Cells
Endocrinology, October 1, 2002; 143(10): 3759 - 3765.
[Abstract] [Full Text] [PDF]

Home page
 Exp. Biol. Med.Home page
D. Cracchiolo, J. W. Swick, L. McKiernan, E. Sloan, S. Raina, C. Sloan, and D. L. Wendell
Estrogen-Dependent Growth of a Rat Pituitary Tumor Involves, But Does Not Require, a High Level of Vascular Endothelial Growth Factor
Experimental Biology and Medicine, July 1, 2002; 227(7): 492 - 499.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
S. Vidal, R. V. Lloyd, L. Moya, B. W. Scheithauer, and K. Kovacs
Expression and Distribution of Vascular Endothelial Growth Factor Receptor Flk-1 in the Rat Pituitary
J. Histochem. Cytochem., April 1, 2002; 50(4): 533 - 540.
[Abstract] [Full Text] [PDF]

Home page
 Endocr. Rev.Home page
N. Ben-Jonathan and R. Hnasko
Dopamine as a Prolactin (PRL) Inhibitor
Endocr. Rev., December 1, 2001; 22(6): 724 - 763.
[Abstract] [Full Text] [PDF]

Home page
 Exp. Biol. Med.Home page
X. Long, K. A. Burke, R. M. Bigsby, and K. P. Nephew
Effects of the Xenoestrogen Bisphenol A on Expression of Vascular Endothelial Growth Factor (VEGF) in the Rat
Experimental Biology and Medicine, May 1, 2001; 226(5): 477 - 482.
[Abstract] [Full Text]


HOME HELP CONTACT US SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2000 by the Society for Endocrinology.