Bombesin (BN) interacts with two mammalian receptor subtypes termed gastrin-releasing peptide (GRP)-preferring (GRP-R) and neuromedin B (NMB)-preferring (NMB-R) that may mediate the satiety action of BN. We examined the feeding behavior of mice that were deficient in the GRP-R (GRP-R KO) to assess the overall contribution of this receptor subtype in the feeding actions of BN-related peptides. GRP-R KO mice failed to suppress glucose intake in response to systemically administered BN and GRP(18-27), whereas both peptides elicited a potent reduction of intake in wild-type (WT) mice. Neither GRP-R KO nor WT mice suppressed glucose intake following NMB administration. Unlike the impaired responses to BN-like peptides, the feeding inhibitory action of cholecystokinin was enhanced in GRP-R KO mice. Consistent with behavioral results, GRP-R KO mice also exhibited a reduction in c-Fos immunoreactivity in the nucleus of the solitary tract (NTS) and paraventricular nucleus (PVN) following peripheral administration of BN. An evaluation of meal patterns showed that GRP-R KO mice ate significantly more at each meal than WT mice, although total 24 h food consumption was equivalent. A long-term analysis of body weight revealed a significant elevation in GRP-R KO mice compared with WT littermates beginning at 45 weeks of age. These data suggest that the GRP-R mediates the feeding effects of BN-like peptides and participates in the termination of meals in mice.

This article has been cited by other articles:

				S. C. Woods and D. A. D'Alessio Central Control of Body Weight and Appetite J. Clin. Endocrinol. Metab., November 1, 2008; 93(11_Supplement_1): s37 - s50. [Abstract] [Full Text] [PDF]

				R. T. Jensen, J. F. Battey, E. R. Spindel, and R. V. Benya International Union of Pharmacology. LXVIII. Mammalian Bombesin Receptors: Nomenclature, Distribution, Pharmacology, Signaling, and Functions in Normal and Disease States Pharmacol. Rev., March 1, 2008; 60(1): 1 - 42. [Abstract] [Full Text] [PDF]

				N Hoggard, S Bashir, M Cruickshank, J D B Miller, and J R Speakman Expression of neuromedin B in adipose tissue and its regulation by changes in energy balance J. Mol. Endocrinol., September 1, 2007; 39(3): 199 - 210. [Abstract] [Full Text] [PDF]

				O. Patel, C. Dumesny, A. Shulkes, and G. S. Baldwin C-Terminal Fragments of the Gastrin-Releasing Peptide Precursor Stimulate Cell Proliferation via a Novel Receptor Endocrinology, March 1, 2007; 148(3): 1330 - 1339. [Abstract] [Full Text] [PDF]

				T. A. Lutz Hunger and satiety: one brain for two? Am J Physiol Regulatory Integrative Comp Physiol, October 1, 2006; 291(4): R900 - R902. [Full Text] [PDF]

				E. E. Ladenheim, M. Emond, and T. H. Moran Leptin enhances feeding suppression and neural activation produced by systemically administered bombesin Am J Physiol Regulatory Integrative Comp Physiol, August 1, 2005; 289(2): R473 - R477. [Abstract] [Full Text] [PDF]

				C. Dumesny, J. C. Whitley, G. S. Baldwin, A. S. Giraud, and A. Shulkes Developmental expression and biological activity of gastrin-releasing peptide and its receptors in the kidney Am J Physiol Renal Physiol, September 1, 2004; 287(3): F578 - F585. [Abstract] [Full Text] [PDF]

				Genetically Modified Animals in Endocrinology Endocr. Rev., August 1, 2004; 25(4): 673 - 677. [Full Text] [PDF]

				F. Maekawa, H.-M. A. Quah, K. Tanaka, and H. Ohki-Hamazaki Leptin Resistance and Enhancement of Feeding Facilitation by Melanin-Concentrating Hormone in Mice Lacking Bombesin Receptor Subtype-3 Diabetes, March 1, 2004; 53(3): 570 - 576. [Abstract] [Full Text] [PDF]