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A tumour of a dysgenetic gonad was removed from an 18-yr.-old girl, who had presented with primary amenorrhoea and poor breast development. Examination showed clitoral hypertrophy, and chromosome analysis showed a normal male 46/XY karyotype. Microscopically, cells of both ovary and testis were recognized in the tumour. Incubation of the tissue with [4-¹⁴C]progesterone in Krebs-Ringer bicarbonate solution showed its capacity to synthesize testosterone and oestradiol-17β. 16-Hydroxyprogesterone was also formed during the incubation.