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To facilitate a study of the metabolism of 17-methyltestosterone (MeT), [1,2-³H] MeT and four unlabelled derivatives of MeT were prepared. This report describes the preparation of these compounds.

[1,2-³H]17-Methyltestosterone. This was prepared by partial tritiation (at the Radiochemical Centre, Amersham, Bucks.) of 17-methyl-17β-hydroxy-androst-1,4-dien-3-one (obtained from Ciba Laboratories, Horsham, Sussex). A hundred mg. of the steroid was dissolved in 5 ml. dioxan and shaken for 5 hr. at 22° in 12 ml. of a tritium-hydrogen mixture (5 C) in the presence of 50 mg. 10 % palladium on charcoal. Eighty mg. of steroid were recovered with an average specific activity of 18 mC/mg. [1,2-³H]MeT was subsequently isolated in a 10–15 % yield by paper chromatography of portions of the tritiated material in the cyclohexane: formamide system (Savard, 1953) and then in the A system of Bush (Bush, 1952).

[1,2-³H]MeT thus obtained