HOME HELP CONTACT US SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by DORRINGTON, J. H. Articles by KILPATRICK, R. Search for Related Content PubMed Articles by DORRINGTON, J. H. Articles by KILPATRICK, R.

Luteinizing hormone (LH) stimulated synthesis of both progesterone and 20-hydroxypregn-4-en-3-one by rabbit ovarian tissue in vitro. Progesterone synthesis was stimulated by nicotinamide adenine dinucleotide phosphate (NADP), but 20-hydroxypregn-4-en-3-one production was only slightly affected by NADP compared with the effect of LH. When NADP was added with glucose-6-phosphate (G-6-P) or 6-phospho-gluconate (6-P-G), no apparent stimulation of progestational steroid synthesis occurred.

Specific activity measurements suggested that stimulation of synthesis was masked by increased conversion of progestational steroids to other products.

When NADP and submaximal concentrations of LH were used together, potentiation rather than addition of effects on progesterone synthesis was found, and addition of effects with supramaximal concentrations of LH. No potentiation was found when NADP was replaced by NADP and G-6-P or 6-P-G, or by NADPH₂. NADP, unlike LH, caused striking stimulation of progesterone synthesis by separated corpora lutea. It is suggested that the present results provide further support for the view that the actions of LH and NADP are related.