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Tanaka & Nakajo (1962) have reported a decrease in the content of vasotocin in the posterior pituitary of hens coincident with oviposition, suggesting that the hormone was released into the blood and causes oviposition. Douglas & Sturkie (1964) assayed vasotocin in the blood of laying hens and found that the concentration of the hormone increased markedly a few minutes before oviposition, returning to resting levels within 10–20 min. after laying. Little is known about the factors involved in the release of vasotocin and this is the principal objective of this study.

Vasotocin was assayed on the isolated frog bladder (Rana catesbiana) by a modification of the method of Sawyer (1960). The movement of water across the bladder wall is proportional to log concentration of hormone acting upon it. Oxytocin (Pitocin assayed against synthetic oxytocin) was used as the standard. Three standard points, 2, 10 and 50 m-u. oxytocin/ml. bath solution,

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