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Since Baulieu (1962) suggested that dehydroepiandrosterone sulphate (DHAS) was a natural adrenal secretory product, several endocrine tissues have been shown to form steroid sulphates. These can be metabolized and still preserve their conjugated form (Calvin & Lieberman, 1964). This report presents evidence for such transformations by polycystic ovarian tissue.

Homogenized tissue, in 3 ml. Krebs—Ringer improved bicarbonate buffer, was incubated with 25 µc [7-³H]DHAS (Radiochemical Centre, Amersham, 605 mc/m-mole) for 2 hr. at 37°, in 95%O₂5%CO₂. Neutral, phenolic and conjugate fractions were obtained after adding carrier steroids to the incubation mixture (Fahmy, Griffiths, Turnbull & Symington, 1968). Complete extraction of free steroids was made before conjugate extraction.

The neutral fraction (45% of recovered radioactivity) was chromatographed on silica gel (TLC) (acetone: methylene chloride, 1:9). Areas were eluted corresponding in position to the added steroids (⁴-androstenedione, DHA, testosterone, ⁵-androstenediol, 16-hydroxy DHA