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Most of the long-acting thyroid stimulator (LATS)-absorbing activity of human thyroid homogenates was shown to be in a soluble fraction containing several components including thyroglobulin, haemoglobulin and serum proteins. Salt precipitation and gel filtration studies indicated that the absorbing activity was not associated with thyroglobulin of high molecular weight, but with a 4s fraction. This 4s fraction has been shown to contain thyroid specific antigens and these may be important in LATS absorption.