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17-Hydroxycorticosterone (F) and corticosterone (B) are the two most abundant steroids in bovine adrenal venous blood (Bush, 1953). The competitive protein-binding assay of Bassett & Hinks (1969) is equally sensitive for both these steroids. This fortuitous advantage, together with the need for only small volumes of blood (< 5 ml.), makes this method of assay ideal for the determination of bovine peripheral plasma corticosteroids.

Ayrshire or Friesian cows, kept under cover and accustomed to being handled, were fed on a diet normal for the Institute herd. A list of the different groups of cows is given in Table 1. All blood samples were taken between 14.00 and 15.00 hr. The sample (about 5 ml.) was taken, on to heparin, from the jugular vein within 30 sec. of approaching the animal. After centrifugation, plasma was stored at –15° until required.

The assay of plasma corticosteroids was essentially as described by Bassett