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In a recent study of renal function in supposedly pregnant rabbits, much time and effort was wasted because about half the mated females failed to become pregnant. On retrospective analysis, the daily sodium excretion was different in the animals which became pregnant as compared with those which failed to become pregnant and which were presumably pseudopregnant. The main difference was apparent on day 4 after mating. We are now using sodium excretion as a simple diagnostic test of pregnancy in rabbits.

Fifteen animals were kept in metabolism cages for 20 days before mating and for the duration of the supposed pregnancy. Only eight of the 15 actually became pregnant. All the urine excreted by each animal each day was collected, its volume measured and its sodium concentration estimated by flame photometry. For each animal the mean daily excretion during the 20-day control period was defined as 100%. Figure 1 shows