HOME HELP CONTACT US SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by PIERREPOINT, C. G. Articles by GRIFFITHS, K. Search for Related Content PubMed Articles by PIERREPOINT, C. G. Articles by GRIFFITHS, K.

Placental tissue was removed from a ewe at 127 days' gestation and, without separating foetal from maternal contributions, incubated as a mince (4 g) with equimolar amounts of [4-¹⁴C]androstenedione (2 µCi, 56 mCi/mmol) and [1,2-³H]epitestosterone (15 µCi, 420 mCi/mmol) at 39·5 °C. The reactions were stopped after 2 h by adding acetone and freezing. The non-radioactive carrier steroids, androstenedione, testosterone, epitestosterone, oestrone, oestradiol-17β, oestradiol-17, testosterone sulphate and epitestosterone sulphate were added in 500 µg amounts.

After mixing, steroids and their conjugates were extracted from the incubation medium with 2 x 100 ml acetone followed by 2 x 100 ml ether: ethanol (3:1, v/v). Nonpolar lipid material was removed by partitioning the pooled, dried extract between 70% (v/v) aqueous methanol and light petroleum (b.p. 40–60 °C). The methanol was evaporated and unconjugated steroids extracted from the aqueous residue with 3 x 30 ml ether before saturating with ammonium