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In rabbits, an anti-pig-luteinizing hormone serum (anti-pig-LH) inhibited coitally-induced ovulation when injected immediately post coitum and this effect persisted for at least 5 days. When administered 90 min after mating, anti-pig-LH had very little inhibitory effect on ovulation and did not prevent the establishment of normal pregnancies. Anti-pig-LH also blocked human chorionic gonadotrophin-induced ovulation in rabbits, evidently by direct cross reaction. Sufficient anti-pig-LH to block ovulation in rabbits inhibited neither ovulation nor conception in four guinea-pigs when injected 0 to 4 days before vaginal opening.