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During short-term incubations of isolated posterior pituitary glands of the mouse, isotopically labelled amino acids were incorporated into protein by the cells of the pars intermedia. Using labelled leucine, 5–10% of incorporated label was found in a protein (P₁), with a molecular weight of about 75000. Protein P₁ could be isolated from both fresh and incubated tissue, and was a normal and indeed major, secretory product of the pars intermedia, constituting more than 50% of the protein present.