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Department of Applied Pharmacology, School of Pharmacy, The Hebrew University, Jerusalem, Israel

(Received 16 June 1976)

Existence of human prolactin (hPRL) in semen, serum and urine has been established in men and women during various physiological and endocrinopathological states (Lyons & Page, 1935; Meites & Turner, 1941; Coppedge & Segaloff, 1951; Segaloff, 1953; Sheth, Mugatwala, Shah & Rao, 1975). Recently a radioimmunological method utilizing NIH hPRL-VLS-2 for iodination and prolactin 72/4/9 as a standard preparation was described: Nader, Mashiter & Joplin (1975) and Sheth et al. (1975) reported that the level of hPRL in semen is four- to sevenfold higher than in serum as assayed in various systems. Due to the low sensitivity of these methods the urine samples had to be pretreated in order to concentrate the hormone, and it occurred to us that developing a sensitive assay method that will enable a simple and direct determination of urinary