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Department of Animal Physiology and Nutrition, Department of Pure and Applied Zoology, University of Leeds, Leeds LS2 9JT, and the *Agriculture Research Council's Poultry Research Centre, King's Buildings, West Mains Road, Edinburgh, EH9 3JS

(Received 31 August 1976)

Prolactin is involved in the control of the oestrous cycle (e.g. rat, Evans, Simpson, Lyons & Turpeinen, 1941; and sheep, Denamur, Martinet & Short, 1973) and probably of the menstrual cycle (McNatty, Sawers & McNeilly, 1974). The present communication describes the changes in the plasma prolactin level during the ovulatory cycle of the chicken.

Twenty-four laying White Leghorn hens, maintained on a lighting schedule of 14 h light: 10 h darkness, were bled by venipuncture from the brachial vein at intervals of 2h. Twelve were sampled for 12 h from the onset of the light phase and twelve for the same period from the beginning of the dark phase. After separation of