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Rowett Research Institute, Bucksburn, Aberdeen, AB2 9SB

(Received 29 October 1976)

Insulin secretion in adult ruminants has been studied extensively in vivo but only one report describes insulin release in vitro (Hertelendy, Machlin, Takahashi & Kipnis, 1968). Insulin release was stimulated by propionate, butyrate, glucose and arginine, but the static incubation system employed precluded an analysis of secretion dynamics. The study reported here was therefore carried out to determine the pattern of in-vitro insulin release from sheep pancreas.

Adult sheep fed on roughage diets were killed by an overdose of sodium pentobarbitone. Pieces of pancreas were excised within 1–3 min, washed in ice-cold Krebs–Ringer bicarbonate buffer, pH 7·4, and cut into 1 mm cubes. Approximately 300 mg of fragments were weighed and placed in a perifusion chamber (Lacy, Walker & Fink, 1972). The perifusion medium was Krebs-Ringer bicarbonate buffer, pH 7·4, containing 5 mM-pyruvate, 5 mM-fumarate, 5 mM-glutamate and 5