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Laboratoire de Physiologie des Poissons and *Laboratoire de Physiologie de la Lactation, I.N.R.A., 78350 Jouy en Josas, France

(Received 11 October 1976)

Fish prolactin bioassays lack specificity (Sage & Bern, 1972), have poor sensitivity, are difficult to use (Ensor & Ball, 1968; Clarke, 1973) and contradictory conclusions have been drawn with them. Nicoll & Bern (1968) obtained no lactogenic effect of teleost pituitary gland in the pigeon-crop and the rabbit mammary gland tests, but Chadwick (1966) demonstrated the occurrence of a mammotrophic effect. Other workers have used heterologous radioimmunoassay (RIA) systems (McKeown & Van Overbeeke, 1972); these RIA systems do not seem to cross-react specifically with fish prolactin (Nicoll, 1975). We present a new approach using rabbit mammary gland prolactin receptors.

The technique used was a modification of the radioreceptor assay (RRA) for lactogenic hormones (Shiu, Kelly & Friesen, 1973). At the beginning of lactation the rabbit was injected with