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Department of Surgery, Ninewells Hospital, Dundee, Angus

(Received 9 November 1976)

The use of antibodies to luteinizing hormone releasing hormone (LH-RH) to study the physiological role of LH-RH would be increased if the antibody could be neutralized at a selected time without otherwise interfering with the hypothalamo-pituitary system. A possible method is to saturate passively transferred antibody in the blood with a fragment of LH-RH which cross-reacts immunologically with the antibody but does not have LH-RH activity.

This study was designed to investigate whether the inhibition of ovulation produced by an antiserum to LH-RH injected into rats at 12.00 h on the day of pro-oestrus could be prevented by subsequent injection of the 3-10 octapeptide fragment of LH-RH on the same day. This fragment is biologically inactive (Rivier, Amoss, Rivier & Vale, 1974). The antiserum was raised in a rabbit to LH-RH (Hoechst) conjugated to bovine serum albumin by carbodiimide