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Institut de Physiologie Générale, Université Louis Pasteur, Rue René Descartes, 67000 - Strasbourg, France

(Received 23 November 1976)

Studies on glucocorticoid binding to the anterior pituitary gland has revealed, in addition to a receptor binding both natural and synthetic steroids, the presence of a compound closely resembling plasma transcortin (Koch, Lutz, Briaud & Mialhe, 1975, 1976; DeKloet & McEwen, 1976). Whether this transcortin-like component, which is not a true receptor and has been shown not to be due to mere blood contamination, is intracellular or bound to the cell membrane is still unclear. In this study, using isolated cells and plasma membrane fractions, we present indirect evidence suggesting attachment of the transcortin-like material to the cellular membrane of the pituitary cells.

Isolated pituitary cells were obtained as described by Portanova, Smith & Sayers (1970), using glands from 1-day adrenalectomized male rats, extensively perfused with ice-cold 0·9% saline through the heart.