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Department of Anatomy, King's College, London, WC2R 2LS

(Received 12 May 1977)

The biosynthetic activity of the pars intermedia of the mouse pituitary gland was investigated by studying the fate of labelled amino acids incorporated into relatively large-molecular-weight products (Thornton & Isherwood, 1975). It was reported that label was actively incorporated in vitro into a protein (referred to as 'labelled P₁') with a molecular weight of about 75 000 (Isherwood & Thornton, 1977). However, more recent observations suggest that 'labelled P₁' is a complex between a protein and a labelled peptide. The purpose of this communication is to give a brief account of some of the findings which led to this conclusion.

Full details of the incubation and cellulose acetate electrophoresis procedures are given in Isherwood & Thornton (1977). Posterior pituitary glands from male, Schofield mice were incubated in media containing [³H]leucine and [³H]phenylalanine (50 µCi/ml). In one experiment, posterior