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Department of Zoology, University of Western Australia, Nedlands, Western Australia 6009, Australia

(Received 13 June 1977)

Buchborn (1955) described a bioassay based on the water-balance response of toads which is simple to perform, has a high sensitivity (0·1 µu./10 toad) and good precision ( = 0·228). However, measurements of the level of antidiuretic hormone (ADH) in the blood obtained with this assay are considerably greater than those reported using other methods (Walker, 1967). We reinvestigated this assay in order to measure seasonal levels of ADH in tammar wallabies.

Neurohypophysectomized toads (Bufo marinus) were starved for 1 week and then held for 3 days in jars containing tap water to a depth of 2 cm. The standard weight was determined (Ruibal, 1962) three times at 1 h intervals. Toads whose standard weight did not vary by more than 0·1 g were injected into the dorsal lymph sac with 5 ml 50