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* Department of Physiology, Michigan State University, East Lansing, Michigan 48824, U.S.A., Departments of Pure and Applied Zoology and Animal Physiology and Nutrition, The University of Leeds, Leeds, LS2 9JT and Department of Biology, Boston University, Boston, Massachusetts 02215, U.S.A.

(Received 24 June 1977)

Recent studies have shown that reptilian hypothalamic extract contains a prolactin releasing factor capable of acting on repitilian pituitary glands when incubated in vitro (Nicoll, Fiorindo, McKenee & Parsons, 1970; Hall, Chadwick & Callard, 1975b). It has also been suggested that steroids may influence the secretion of prolactin in reptiles (Callard, Doolittle, Banks & Chan, 1972). Müller, Sawano & Schally (1967) found evidence for a reptilian growth hormone (GH) releasing factor using a heterologous system. We have examined the control of the secretion of prolactin, GH and luteinizing hormone (LH) from the pituitary gland of the painted terrapin in vitro.

Pituitary glands and hypothalami were removed from