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MRC Unit of Reproductive Biology, 2 Forrest Road, Edinburgh, EH3 9ER and * Agricultural Research Council's Poultry Research Centre, King's Buildings, West Mains Road, Edinburgh, EH9 3JS

(Received 22 August 1977)

The decapeptide luteinizing hormone releasing hormone (LH-RH) stimulates the release of luteinizing hormone (LH) in birds as well as in mammals (Van Tienhoven & Schally, 1972) and a substance immunochemically similar to LH-RH is present in the chicken hypothalamus (Jeffcoate, Sharp, Fraser, Holland & Gunn, 1974). Avian LH-RH has still to be isolated and sequenced, however, and there is some doubt about whether the decapeptide is the naturally occurring LH-RH in the bird (Jackson, 1971).

In the hen, release of LH is induced by the positive feedback action of progesterone (Wilson & Sharp, 1975) which is presumably associated with the release of chicken LH-RH. To gain further information about the identity of chicken LH-RH and to investigate the site

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