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Department of Medicine, Manchester Royal Infirmary, Manchester, M13 9WL

(Received 17 October 1977)

Recently Feldman, Dziak, Koehler & Stern (1975) have detected high-affinity gluco corticoid receptors in cytosol from isolated foetal rat calvarial bone cells. They estimated the binding constant (K_d) to be 7x10^–9 mol/l for [³H]dexame[unk]hasone; however, their estimates were based on a maximum bound: free ratio of only 1%. Their data from cell cultures suggest that such binding may nevertheless be of physiological importance (Chen, Aronow & Feldman, 1977). The very small amount of binding might be due to a low initial concentration of binding sites, to their destruction during or after the trypsin–collagenase digestion, to their high dilution or to a combination of all three factors. We report here a much simpler method whereby we have greatly increased the percentage binding by setting up the incubation in intact calvaria.

Calvaría were dissected from foetal rats (19–21 days

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