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A simplified, more rapid bioassay for the estimation of somatomedin activity, based on the porcine costal cartilage bioassay, is described. A micro-scale system based on the porcine costal cartilage assay is also described. These assay systems showed no decrease in sensitivity or precision, compared with other methods, as a result of the modifications. The assays have been used for routine hospital laboratory determinations, for the estimation of somatomedin activity in normal adolescent children of both sexes and in clinical studies of scoliotic, acromegalic and hypopituitary patients.