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Abteilung für Klinische Endokrinologie, Department für Innere Medizin und Zentrales Tierlabor, Medizinische Hochschule, 3000 Hannover 61, Federal Republic of Germany

(Received 8 May 1978)

The application of a blood sampling technique which by itself has little or no influence on the concentrations of hormones in the circulation of the rat is of utmost importance in experimental hormone research. It has recently been demonstrated that the type of anaesthesia and probably also the site of withdrawal of blood from the body may become critical parameters when the determination of unaltered hormone levels is required (Döhler, von zur Mühlen, Gärtner & Döhler, 1977b). That the site of blood sampling may act as an interfering parameter is questionable, since cage transport, handling and possible differences in the intensity of anaesthesia may also influence the variability of individual hormone levels (Baldwin, Colombo & Sawyer, 1974; Krulich, Hefco, Illner & Read, 1974; Euker, Meites &

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