HOME HELP CONTACT US SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by FORSYTH, I. A. Articles by NICOLL, C. S. Search for Related Content PubMed Articles by FORSYTH, I. A. Articles by NICOLL, C. S.

Ovine prolactin, labelled with ¹²⁵I by either lactoperoxidase or a mild chloramine T method, bound to receptors from the pigeon crop sac mucosa cells of prolactin-injected pigeons. Binding was demonstrated in a crude homogenate of mucosal cells removed from the crop by scraping and in a subcellular fraction in which 5'-nucleotidase activity was enhanced two- to threefold. The binding was specific, dependent on time, temperature and the concentration of receptors and had a dissociation constant of 7 x 10^–10 mol/l. The binding capacity of the crop tissue was 71 fmol/mg membrane protein. Nine purified preparations of prolactin from four species were assayed by local pigeon crop sac bioassay and by radioreceptor assay. The two methods were highly correlated (r = 0·934). The regression equation was radioreceptor assay = 1·22 bioassay–0·18 indicating a 1: 1 correspondence between the two methods for prolactin purified from sheep, rat, horse and pig anterior pituitary glands.

This article has been cited by other articles:

				B. White and C. Nicoll Prolactin receptors in Rana catesbeiana during development and metamorphosis Science, May 25, 1979; 204(4395): 851 - 853. [Abstract] [PDF]