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There was no loss of biological activity in vitro when the 1st International Reference Preparation (IRP) for human pituitary gonadotrophins [FSH and LH/interstitial cell stimulating hormone (ICSH) for bioassay] code no. 69/104 and the 1st IRP for human pituitary LH/ICSH [for immunoassay] code no. 68/40 were stored for 1 year at –70 °C in a buffered 0·8% saline solution containing 1% bovine plasma albumin (BPA). However, storage of the 69/104 preparation at –20 °C in either 0·1 or 1% BPA, or at –70 °C in the presence of 0·1% BPA showed a small but significant decrease ( 10%) in activity over the same period. It is, therefore, advantageous to store these reference preparations at –70 °C in the presence of 1% BPA.