HOME HELP CONTACT US SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by ROY, E. J. Articles by McEWEN, B. S. Search for Related Content PubMed Articles by ROY, E. J. Articles by McEWEN, B. S.

Oestrogen receptors were measured by an exchange assay in cell nuclei of the hypothalamus–preoptic area-amygdala (HPA) of ovariectomized-adrenalectomized (OVX-ADX) rats following an intravenous injection of oestradiol or an antioestrogen, CI-628. Receptors were translocated to the nucleus by both compounds. Receptors translocated by the antioestrogen were specific for oestrogens; testosterone, corticosterone and progesterone were not bound by these receptors. Several properties of antioestrogen– and oestradiol–receptor complexes were compared.

The time-course in cell nuclei in vivo showed that receptors were still present in HPA nuclei 24 h after administration of CI-628 but not 24 h after oestradiol. This prolonged increase of nuclear receptors after the antioestrogen treatment was attributed to the continued presence of CI-628 and its metabolites in plasma.

The maximum level of receptors produced in the HPA cell nuclei following CI-628 treatment was lower than the peak level of nuclear receptors following oestradiol. The dissociation rate in vitro of nuclear antioestrogen–receptor complexes formed in vivo was more rapid at 0 °C than that of nuclear oestradiol–receptor complexes. This property may be related to the lower peak level of binding after CI-628 treatment and to the inhibitory actions of antioestrogens.

This article has been cited by other articles:

				E. Roy and M. Wilson Diurnal rhythm of cytoplasmic estrogen receptors in the rat brain in the absence of circulating estrogens Science, September 25, 1981; 213(4515): 1525 - 1527. [Abstract] [PDF]