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The effects of 2-hydroxyoestradiol (2OH-OE2), dopamine, oestradiol-17β and 2OH-OE2 plus dopamine on prolactin and LH release from the male rat pituitary gland were examined in vitro.
2-Hydroxyoestradiol reduced prolactin secretion by 51% at 10–10 mol/l and by 34% at 10–7 mol/l, while oestradiol-17β had no effect at these doses. Dopamine alone (5 x 10–7 mol/l) decreased prolactin released by 58%, 2OH-OE2 plus dopamine produced a similar inhibition of 60%.
No significant effect on LH release was observed throughout.
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