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Individual chorion taken from cows in early pregnancy (days 27, 44, 45 and 49) was incubated with [¹⁴C]androstenedione in short-term incubation in vitro. After preliminary extraction, separation and purification, metabolites were identified by recrystallization with authentic unlabelled steroids to constant specific activity. Major metabolites identified were 5β-androstane-3,17-dione (5β-androstanedione) and 5β-androstan-3-ol-17-one (aetiocholanolone) while minor metabolites were identified as 5β-androstane-3,17β-diol and 5β-androstan-17-ol-3-one. There was no ¹⁴C-labelled oestradiol or oestrone detectable in the medium either as free oestrogens or as sulphates.