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This Article Accepted manuscript (PDF) All Versions of this Article: JOE-08-0082v1 198/2/339    most recent Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Mondal, M. Articles by Nakazato, M. Search for Related Content PubMed PubMed Citation Articles by Mondal, M. Articles by Nakazato, M.

M Mondal, Internal Medicine, University of Miyazaki, Miyazaki, Japan
K Toshinai, Internal Medicine, University of Miyazaki, Miyazaki, Japan
H Ueno, Internal Medicine, University of Miyazaki, Miyazaki, Japan
K Koshinaka, Internal Medicine, University of Miyazaki, Miyzazaki, Japan
M Nakazato, Miyazaki, Japan

Correspondence: Muhtashan Mondal, Email: ms_mondal2000{at}yahoo.com

Abstract

Obestatin is a 23-amino acid peptide, initially isolated from rat stomach as an endogenous ligand for the orphan G protein-coupled receptor GPR-39. Obestatin is derived from proteolytic cleavage of a 117-amino acid precursor, preproghrelin. Ghrelin increases food intake, body weight, and gastric emptying, whereas obestatin has the opposite effects. In this study, we characterized obestatin in both rat and human stomach, and investigated the peptide|'s effect on feeding behavior. Using reversed-phase high performance liquid chromatography coupled with radioimmunoassays specific for rat and human obestatin, we detected a very small amount of obestatin, compared with ghrelin, in the gastric fundi. The ratios of obestatin to ghrelin are 0.0039% and 1.94%, respectively, in the rat and human gastric fundi. In humans, plasma obestatin accounted for 5.21% of the ghrelin concentration, whereas it was undetectable in rat plasma. Plasma ghrelin concentration decreased after a meal in normal subjects, whereas obestatin concentration did not change. When administered centrally or peripherally, obestatin did not suppress food intake in either free-feeding or fasted rodents. Administration of obestatin did not antagonize ghrelin-induced feeding. These findings indicate that obestatin is present at very low levels compared with ghrelin in both rat and human, and has no acute effect on feeding behavior.